Accurate Calibration and Uncertainty Estimation of the Normal Spring Constant of Various AFM Cantilevers

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Accurate Calibration and Uncertainty Estimation of the Normal Spring Constant of Various AFM Cantilevers

Measurement of force on a micro- or nano-Newton scale is important when exploring the mechanical properties of materials in the biophysics and nanomechanical fields. The atomic force microscope (AFM) is widely used in microforce measurement. The cantilever probe works as an AFM force sensor, and the spring constant of the cantilever is of great significance to the accuracy of the measurement re...

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An analytic model for accurate spring constant calibration of rectangular atomic force microscope cantilevers

Spring constant calibration of the atomic force microscope (AFM) cantilever is of fundamental importance for quantifying the force between the AFM cantilever tip and the sample. The calibration within the framework of thin plate theory undoubtedly has a higher accuracy and broader scope than that within the well-established beam theory. However, thin plate theory-based accurate analytic determi...

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Uncertainty Quanti cation in Calibration of AFM Probes Due to Non-uniform Cantilevers

For more than two decades, the Atomic Force Microscope (AFM) has provided valuable insights in nanoscale phenomena, and it is now widely employed by scientists from various disciplines. AFMs use a cantilever beam with a sharp tip to scan the surface of a sample both to image it and to perform mechanical testing. Since the AFM measures the de ection of the probe beam, one must rst nd the spring ...

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Spring constant calibration of atomic force microscope cantilevers of arbitrary shape.

The spring constant of an atomic force microscope cantilever is often needed for quantitative measurements. The calibration method of Sader et al. [Rev. Sci. Instrum. 70, 3967 (1999)] for a rectangular cantilever requires measurement of the resonant frequency and quality factor in fluid (typically air), and knowledge of its plan view dimensions. This intrinsically uses the hydrodynamic function...

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ژورنال

عنوان ژورنال: Sensors

سال: 2015

ISSN: 1424-8220

DOI: 10.3390/s150305865